|  | Breakthrough initiation1
|
---|
Nutrient medium | Organism | TBF Treatment: GLUT | BBF Treatment: GLUT | TBF Treatment: AHP | BBF Treatment: AHP |
---|
High2
|
E. faecalis
| 15 | 7 | 24 | 12 |
 |
P. aeruginosa
| 6 | 5 | 9 | 8 |
 |
C. albicans
| 9 | 5 | 15 | 7 |
Low3
|
E. faecalis
| 30 | 15 | NBD4
| NBD |
 |
P. aeruginosa
| 18 | 12 | NBD | NBD |
 |
C. albicans
| 24 | 13 | NBD | NBD |
- The day (in TBF) or cycle (in BBF) when breakthrough growth was first detected in traditional biofilm or buildup biofilm formed by repetitive exposure to drying, HLD, and reseeding. Results are an average of 9 replicates; SD ≤ 10%.
-
1 For TBF, the breakthrough time is recorded in days. For BBF, the breakthrough time is recorded in cycles (of a 2-day cycling period).
-
2 High nutrient media (ATS (for TBF) or Enzymatic detergent (for BBF))
-
3 Low nutrient media (Water)
-
4 NBD: no breakthrough detected; limit of detection for viability counting is 10 cfu/peg