A Schistosoma japonicumchimeric protein with a novel adjuvant induced a polarized Th1 immune response and protection against liver egg burdens

Table 2 Effects of immunization with the SjGP-3 formulated by various adjuvants on induction of IgG and protective efficacy against challenge in mice

Groups ^a		IgG titers ^b	Worm burdens Mean ± SE	%Worm reduction	Liver eggs (epg) Mean ± SE ^c	% Egg reduction
Exp1	SjGP-3/CFA	1,890,000	19.8 ± 0.9	41.1*	20,900 ± 1,947	26.6*
	SjGP-3/ISA206	1,290,000	23.4 ± 1.4	30.4*	21,200 ± 2,307	25.6*
	SjGP-3/IMS1312	680,000	25.3 ± 1.2	24.7*	24,400 ± 2,604	14.4
	SjGP-3/ISA70M	1,790,000	27.8 ± 1.2	17.3*	15,100 ± 1,578	47.0*^,#
	Control	< 500	33.6 ± 1.0	/	28,500 ± 2,777	/
Exp2	SjGP-3/CFA	1,831,000	20.9 ± 1.2	38.2*	26,900 ± 2,557	29.6*
	SjGP-3/ISA206	1,160,000	24.0 ± 1.6	29.0*	28,300 ± 1,808	25.9*
	SjGP-3/IMS1312	783,000	24.7 ± 1.9	26.9*	31,500 ± 2,593	17.5
	SjGP-3/ISA70M	1,841,000	26.0 ± 0.9	23.1*	19,000 ± 1,817	50.3*^,#
	Control	< 500	33.8 ± 0.8	/	38,200 ± 3,428	/

^a: The number of mice per group is from 8 to 10; ^b:Cut-off value was determined based on the pre-immunized geometric mean and three standard deviations. ^c: Number of liver eggs was calculated as egg numbers per gram of liver (epg). *: p < 0.05 when compared with PBS control group. ^#: p < 0.05 when compared with other adjuvant groups. Each value was derived from the arithmetic means of individuals in the group.

ISSN: 1471-2334