Effect of different adjuvant on liver egg numbers per female worm. Liver egg numbers per female worm was measured by total number of liver eggs/total number of paired female worm of individual mouse. The reduction rate of liver eggs per female worm was calculated according to the following formula: (1- mean number of liver eggs per female worm in immunized mice/mean number of liver eggs per female worm in control mice) × 100. There was a significant reduction of the number of liver eggs per female in ISA 70M groups at 34.5–37.2%, while the number of liver eggs per female was not significantly reduced in CFA, ISM 1312 and ISA 206 adjuvant groups (Exp 1: 0.6%, 1.9% and -3.2%; Exp 2: -4.1%, -3.4% and -12.5%, respectively). The number of mice per group is from 8 to 10. * p < 0.05 when compared with PBS control group. This picture shows one experiment representative of two independent trials with similar results.