Analysis of cytokine production. Spleen cells were harvest from mice immunized with the SjGP-3 formulated by various adjuvants (three mice each group) on week 9 (before challenge), 12 (three weeks after cercarial challenge) and 15 (six weeks after cercarial challenge), and stimulated in vitro with anti-CD3, SjGP-3 protein or medium in duplicate wells respectively. The IL-4 and IFN-γ production in the culture supernatants of spleen cells was analyzed by ELISA in triplicate wells. Data correspond to the mean values ± standard deviation. (A) Week 9. (B) Week 12. (C) Week 15. * p < 0.05, ** p < 0.01 when compared with PBS control group.