SjGP-3-specific immunoglobulin subtypes (IgG1, IgG2a and IgE). Groups of mice were immunized with SjGP-3 formulations. Serum was collected after three time immunization. IgG1, IgG2a and IgE titers were detected by ELISA in triplicate wells respectively and the IgG2a to IgG1 ratio was calculated. (A) Anti-SjGP-3 antibody titers. (B) IgG2a/IgG1 ratio. * p < 0.05 when compared with other groups. This picture shows one experiment representative of two independent trials with similar results.