Figure 1

Schematic representation of chimeric gene constructs, recombinant proteins and interaction of immune sera with native proteins. (A) Schematic representation of SjGP-1, SjGP-2, SjGP-3, and SjGP-4 gene construction. Paramyosin was divided into four overlapping fragments and the length of amino acid (Aa) sequence of each fragment is indicated. Each paramyosin fragment was fused to Sj26GST to generate chimeric gene via a hinge (H) consisting of six amino acid residues that were the thrombin recognition site. (B) Purity analysis of purified chimeric proteins by SDS-PAGE. Lane M: molecular mass maker; lanes 1 to 4: purified SjGP-1, SjGP-2, SjGP-3, and SjGP-4 protein, respectively. (C) Interaction of immune sera against the individual chimeric proteins with soluble worm antigen preparation (SWAP) by Western Blot. The SWAP material was separated by SDS-PAGE and analyzed by Western Blot using either immune sera to the individual chimeric proteins or negative control sera. Lane M: molecular mass maker; lanes 1 to 4: sera of mice immunized with SjGP-1, SjGP-2, SjGP-3, and SjGP-4, respectively; lane 5: serum from PBS control mice. (D) Interaction of immune sera against the individual chimeric proteins with purified native Sj26GST. The purified native Sj26GST was separated by SDS-PAGE and analyzed by Western Blot using either immune sera to individual chimeric protein or negative control sera. Lane M: molecular mass maker; lanes 1 to 4: sera of mice immunized with SjGP-1, SjGP-2, SjGP-3, and SjGP-4, respectively; lane 5: serum from PBS control mice.