Sensitivity and specificity of RT-LAMP system. a Sensitivity of RT-LAMP system for purified viral RNA of PV(Sabin) strains. b Kinetics of the detection in RT-LAMP system. The average time required for the detection of the signals is shown for each numbers of the copies. c Sensitivity and specificity of RT-LAMP system for enterovirus. Cell culture supernatants of the cells infected with enteroviruses were used for the detection of the viral RNA by RT-LAMP system. The numbers in the parenthesis show the titre of virus (CCID50) included in the RT-LAMP reactions. The numbers of copies of the viral genome per CCID50 are also shown for each virus. NT, not tested. d Sensitivity and specificity of RT-LAMP system for the viral RNA purified from stool extracts of AFP cases.