| |
pg/ml†
|
|---|
| | |
Patient cells††
|
|---|
|
Cytokine
|
Healthy controls
|
Before MTX
|
After MTX
|
|---|
|
IFN-γ
|
0-10
|
13
|
<10
|
|
IL-1β
|
0-10
|
1 100
|
2
|
|
IL-6
|
0-35
|
9 482
|
864
|
|
IL-8
|
150-2000
|
1 4795
|
1094
|
|
IL-10
|
0-10
|
425
|
4
|
|
TNF-α
|
0-60
|
92
|
<10
|
-
† Cytokine levels in supernatants from cells incubated 48 h in medium alone and quantified using FlowCytomix Human Th1/Th2 Kit as recommended (Bender Medsystems, Vienna, Austria, http://www.bendermedsystems.com).
-
†† No cytokine was detected in serum. IFN-α, IL-2, IL-4, IL-5 and IL-12 were not detected in these samples.
