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Table 1 Protection of mice immunized with HA DNA vaccine against lethal H5N1 virus challengea

From: A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice

   Lung virus titerb
(log10 TCID50/ml)
Survival rate
(No. of survivors/no. tested)
Plasmid DNA Dose
(μg)
3 days 5 days 7 days 3 days 5 days 7 days
HA
DNA
10 6.25 ± 0.35 4.80 ± 0.28c 4.25 ± 0.35c 0/12 0/12 6/12
  50 5.75 ± 0.35 4.55 ± 0.07c 4.00 ± 0.70c 0/12 4/12 10/12d
  100 5.75 ± 0.35 4.55 ± 0.07c 3.25 ± 0.35c 0/12 6/12 12/12d
Control 6.25 ± 0.35 0/12
  1. a Mice were immunized once with HA DNA 3, 5 and 7 days, respectively, before the challenge and at various dosages. Then all the mice were challenged under anesthesia with 5 LD50 of homologous A/Chicken/Henan/12/2004(H5N1) virus. Three days after the viral challenge, the mice were sacrificed. The trachea and lungs were taken out and washed twice by injecting 2 ml of PBS containing 0.1% BSA. The bronchoalveolar wash was used for virus titration after removing cellular debris by centrifugation. The survival of mice 3 weeks after the challenge was measured.
  2. bValues represent means ± SD of 4 mice from each group.
  3. c Significantly different from the control groups (p < 0.05) by ANOVA.
  4. d Significantly different from the control groups (p < 0.05) by Fisher's exact test.