Figure 4
Plasmid profiles of parent and cured strains: M: Molecular weight marker (WBG 4483; Only closed circular DNA are labelled); Lanes 1–3: High-level mupirocin-resistant strains: Lane 1 – A15 (South Africa), Lane 2 – 35 IBA (Nigeria), Lane 3 – P1929 (South Africa); Lane 4: A15 parent strain; Lane 5 – A15cA (cured strain of A15 – resistant to mupirocin, susceptible to erythromycin – 38 kb plasmid encoding high-level mupirocin resistance); Lane 6 – A15cD (cured strain of A15 – susceptible to mupirocin, resistant to erythromycin – 2.3 kb plasmid); Lane 7 – 35 IBA parent (35 kb plasmid encoding high-level mupirocin resistance); Lane 8 – 35 IBA (CUR) – cured strain of 35 IBA (susceptible to mupirocin, resistant to tetracycline).