Expression of tTG and ubiquitin in the brains of
infected mice. (A) Protein levels of tTG and ubiquitin (arrow head) and ubiquitylated protein (thick arrows) expression in the brains of infected mice from 3 dpi to 8 wpi were assessed by Western blotting. (B) Relative times were generated as described in Fig. 5B. The error bars indicate the S.D. and the superscripts represent significant differences to the control; +
P < 0.01, #
P < 0.001. (C) The expression levels of tTG in the brains of infected mice from 3 dpi to 8 wpi were assessed by RT-PCR. (D) The relative amount of tTG mRNA were calculated based on the optical density relative to that of the GAPDH. The error bars indicate the S.D. and the superscript represents significant differences from the control; +
P < 0.01. Three to eight mice per group were examined.