Effects of PDTC and PD98059 on HIV-1 Tat induce destruction of barrier in RPE. D407 were treated in 100 nM HIV-1 Tat and Hi-Tat for 24 h with or without pretreatment with the PDTC (100 μM) and PD98059 (30 μM) for 1.5 h. The TER and paracellular permeability were determined as previously described. Data are the mean ± S.D. of 3 independent experiments (*P < 0.05 vs. control; §
P < 0.05 vs. HIV-1 Tat protein alone).