Figure 8

Effects of HIV-1 Tat on NF-κB DNA binding activity in in RPE. D407 were treated with HIV-1 Tat protein (100 nM) for 1, 2, and 4 h. Nuclear extracts were prepared. DNA binding activity was determined using 2 μg of nuclear protein as described in MATERIALS AND METHODS. To determine the effects of PDTC and SB-203580 on HIV-1 Tat protein induced NF-κB activation in RPE, D407 were treated in 100 nM HIV-1 Tat and Hi-Tat for 4 h with or without pretreatment with the PDTC (100 μM) and PD98059 (30 μM) for 1.5 h. Values shown represent the mean ± S.D. of three independent assays or experiments (*P < 0.05 vs. control; ^§ P < 0.05 vs. HIV-1 Tat protein alone).