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Table 4 False negative cases.

From: Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid

Pt.

BAL Culture

BAL Histology

Clinical Diagnosis

Human DNA (ng per reaction)

CT scan results and other clinical information

8

negative

negative

IPA

0.23 from 1 BAL

No evidence of IPA in BAL; patchy bilateral infiltrates; lung biopsy 1 week later confirmed IPA by culture and histology

9

positive

negative

IPA DAH Staphylococcus pneumonia

33 and 72.5 from 2 BALs

1 CFU A. fumigatus in BAL fluid; patchy nodular infiltrates on CT; on ambisome for 10 days prior to bronchoscopy

10

positive

negative

IPA Legionella CMV pneumonia

10 to 218 from 4 BALs

1 CFU A. niger in BAL with CT scan showing halo sign, IPA confirmed at autopsy

  1. Additional information about false negative (FN) cases as identified by qPCR. Pt.: patient; DAH: Diffuse alveolar hemorrhage; CMV: Cytomegalovirus.