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Table 2 Summary of diagnostic performance in the detection of IPA.

From: Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid

Diagnostic assay BAL fraction Sensitivity (%) Specificity (%) Positive Predictive Value (%) Negative Predictive Value (%) Positive Likelihood Ratio Negative Likelihood Ratio
qPCR, 13 fg pellet 76.9 (50 – 92) 87.7 (79 – 93) 58 94 8.63 (3.2 – 11.9) 0.33 (0.1 – 0.7)
qPCR, 13 fg supernatant 40 (17 – 69) 97.3 (86 – 99) 80.02 85.70 14.8 (1.9 – 118.1) 0.62 (0.37 – 1)
Culture whole (unfractionated) 84.6 (58 – 96) 100 (95 – 100) 100 97.6 infinity 0.15 (0.04 – 0.6)
Histology whole (unfractionated) 53.8 (29 – 77) 100 (95 – 100) 100 93.1 infinity 0.46 (0.3 – 0.8)
Culture or Histology whole (unfractionated) 85.7 (60 – 96) 100 (95 – 100) 100 97.7 infinity 0.99 (0.04 – 0.5)
  1. The range of values within brackets are estimated for a confidence interval of 95%. Thirteen femtograms (fg) of Aspergillus DNA was selected as our threshold for a positive PCR assay result, and is approximately equal to 1/3rd of an Aspergillus fumigatus genome. The supernatant fraction of the BAL was assayed for the first 48 episodes.