Figure 1

Panel A shows representative FACS contour plots gated on CD3+ lymphocytes showing CD4+ and CD8+ proliferative responses to no stimulation (negative control), SEB (superantigen), CMV (recall antigen) and Gag p55 peptide pool (HIV-specific antigens) for a patient with a CD4 count change during TI that was lower than the median for the group (Patient #3) in the left hand panels and a patient with a change in CD4 count during TI that was higher than the median for the group (Patient #1) in the right hand panels. The number in the box of each contour plot is the percentage of CFSE^lo cells, which is an indication of the level of proliferation that has occurred in response to that stimulus. In Panel B, results from all patients are plotted for which proliferation data was available. The percentage of CFSE^lo cells following stimulation is plotted on the y-axis and CD4 count change from baseline to nadir during TI on the x-axis. The left hand panels show the percentage of CFSE^lo cells in the CD4+ T cell compartment and the right hand panels the percentage of CFSE^lo cells in the CD8+ T cell compartment. The upper panels show proliferation to CMV lysate (n = 12) and the bottom panels to the HIV Gag p55 peptide pool (n = 13). Proliferation to CMV and the Gag p55 peptide pool was assessed on baseline time points. The line in each graph represents a trendline through the data points. Spearman nonparametric correlation tests were used to test the significance of the association between these parameters; and p values <0.05 were considered significant.