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Table 2 Ct-values of CT- and (+/-) LGV- positive specimens tested in real-time PCRs in dependence on multiplexing

From: Rapid Detection of Chlamydia trachomatisand Typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR

Sample

Specimen

CT (+/-IK_C)a

LGV (+/-IK_C)a

IK_C

L1 (+/-L2)

L2 (+/- L1)

L3 (+/-GAPDH)

L1

DNA

26/26

28/27

38

28/28

42/-

-/-

L2

DNA

27/26

27/27

42

-/-

28/30

-/-

L3 (dilut.)

DNA

34/34

33/35

32

-/-

-/-

34/35

04/256

Ocular

34/36

-/-

37

42/-

-/-

42/-

04/270

Urethral

-/-

-/-

36

40/-

-/-

-/39

04/1180

Rectal

26/26

-/-

36

-/-

-/-

-/-

06/3013

Rectal

35/36

-/-

36

-/-

-/-

-/39

04/234

Rectal

23/23

25/24

-

-/-

26/28

-/-

04/227

Rectal

32/31

34/33

34

-/-

35/36

-/-

04/261

Rectal

31/30

34/33

-

-/-

38/37

-/-

04/985

Rectal

31/30

34/32

34

-/-

34/32

40/-

05/697

Rectal

29/29

31/31

36

-/-

33/33

36/35

06/567

Urethral

nt/34

nt/34

36

-/-

36/37

-/-

06/1507

Rectal

28/28

31/31

35

-/-

31/34

-/39

  1. a CT/LGV-real-time PCR with (+) and without (-) addition of the internal control plasmid IK_C
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BMC Infectious Diseases

ISSN: 1471-2334

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