Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA

Table 3 Dot- blot ELISA of class 5 using Monoclonal antibodies for N. meningitidis.

		1 week			4 weeks	1 year	4oC	13 years	4 weeks	RT
Mabs		dilution(a)	dilution(b)			dilution(a)	dilution(b)		dilution(a)	dilution(b)
P5.3	WRAIR3BH-C7	1.50,000	1.50,000	1:10,000	1:10,000	1:10,000	1:10,000	1:1000	1:10,000	1:10,000
P5.4	WRAIR1BG11	1.50,000	1.50,000	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500
P5.5	WRAIR3DH-F5G9	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:2,500	1:2,500	1:2,500
P5.8	IALC14F10Br2	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:2,500	1.50,000	1.50,000
P5.9	7F11B5Br3	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:2,500	1.50,000	1.50,000

WRAIR = Walter Reed Army Institute of Research (USA).
NVI = Netherlands Vaccine Institute (previously the RIVM).
Adolfo Lutz Institute (IAL). The Mabs were prepared in Immunology Section by EN De Gaspari.
RT = Room Temperature (30 days).
dilution(a) (Mab) freezer -20°C.
dilution(b) (Mab) eluted of dried filter paper.
Mabs = monoclonal antibodies

ISSN: 1471-2334