Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA

Ferraz, Aline S; Belo, Elza FT; Coutinho, Ligia MCC; Oliveira, Ana P; Carmo, Andréia MS; Franco, Daniele L; Ferreira, Tatiane; Yto, André Y; Machado, Marta SF; Scola, Monica CG; De Gaspari, Elizabeth

doi:10.1186/1471-2334-8-30

Table 2 Dot-blot ELISA of serotypes and subtypes using Monoclonal antibodies for N. meningitidis.

From: Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA

A
				1 week		4 weeks		1 year		13 years	4 weeks	RT
Specificity	Mabs		isotypes	dilution(a)	dilution(b)	dilution(a)	dilution(b)	dilution(a)	dilution(b)	dilution(b)	dilution(a)	dilution(b)
P2.2a	1-1-P2a	WRAIR 5D4-5	IgG3	1:100,000	1:100,000	1:100,000	1:100,000	1:100,000	1:100,000	1:5000	1:100,000	1:100,000
P2.2b	3-1-P2b	WRAIR 2H10-2	IgG3	1:5000	1:5000	1:5000	1:5000	1:5000	1:5000	1:500	1:5000	1:5000
P2c	5D9-5.6(5-1-P2c)	WRAIR	n/d	1:500.000	1:500.000	1:500.000	1:500.000	1:500.000	1:500.000	1:5000	1:500.000	1:500.000
P3.4	15-1-P4	WRAIR 5DC4C8G8	IgG2b	1:2000	1:2000	1:2000	1:2000	1:2000	1:2000	1:500	1:2000	1:2000
P3.15	2-1-P15	WRAIR 8B5-5-G9	IgG2a	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:5000	1.50,000	1.50,000
P3.21	14-1-P21	WRAIR 6B11-F2-C11	IgG2a	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:5000	1.50,000	1.50,000
B
P1.1		NVI MN14C2.3	IgG2a	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.2		NVI MN16C13F4	IgG2a	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.3	12-1-P1.3	WRAIR 5G8B2F9	IgG2a	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:50	1:1,000	1:1,000
P1.4		NVI MN20B9.34	IgG2a	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:100	1:2,500	1:2,500
P1.5		NVI MN22A9.19	IgG2a	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:2,500	1:100	1:2,500	1:2,500
P1.6		NVI MN19D6.13	IgG3	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.7		NVI MN14C11.6	IgG2a	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:100	1:1,000	1:1,000
P1.9		NVI MN5A10F	IgG2a	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.9		IAL5F81A4		1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.10		NVI MN20F4.17	IgG2b	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:500	1:1,000	1:500
P1.12		NVI MN21A7.10	IgG3	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.13		NVI MN25H10.75	IgG2a	1:500	1:500	1:500	1:500	1:500	1:500	1:100	1:500	1:500
P1.14		NVI MN21G3.17	IgG3	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:1,000	1:100	1:1,000	1:1,000
P1.15		NVI MN3C5C	IgG3	1:500	1:500	1:500	1:500	1:500	1:500	1:500	1:500	1:500
P1.16		NVI MN5C11G	IgG2b	1:500	1:500	1:500	1:500	1:500	1:500	1:500	1:500	1:500
P1.19	2-1-P1.19	WRAIR 7A2-11	IgG3	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1.50,000	1:500	1.50,000	1.50,000

WRAIR = Walter Reed Army Institute of Research (USA).
NVI = Netherlands Vaccine Institute (previously the RIVM).
Adolfo Lutz Institute (IAL). The Mabs were prepared in Immunology Section by EN De Gaspari.
RT = Room Temperature (30 days).
dilution(a) (Mab) freezer -20°C.
dilution(b) (Mab) eluted of dried filter paper.
Mabs = monoclonal antibodies.
A = Serotyes.
B = Subtypes

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ISSN: 1471-2334

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