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Table 3 Comparison of different systems for the detection of CHPV.

From: Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura Virus

Host/Method

PI (hours)

Limit of detection (≥) (Replicates +ve for CHPV/Total no of replicates)

RD cells

48

1.2 × 102 (2/4)

PS cells

48

1.2 × 103 (2/4)

Vero Cells

48

1.2 × 103 (3/4)

Sand fly Cell Line

48

1.2 × 102 (3/4)

Chick Embryo

48

1.2 × 102 (2/4)

Mice

48

1.2 × 102 (2/4)

Real Time One step RT-PCR

-

1.2 × 100 (3/3)

Nested RT-PCR

-

1.2 × 100 (3/3)

  1. Limit of detection has been scored positive when ≥ 50% replicates showed CHPV by IFA, plaque formation/death or PCR amplification. Limit of detection has been presented in terms of PFU/ml.