Skip to main content

Table 3 Comparison of different systems for the detection of CHPV.

From: Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura Virus

Host/Method PI (hours) Limit of detection (≥) (Replicates +ve for CHPV/Total no of replicates)
RD cells 48 1.2 × 102 (2/4)
PS cells 48 1.2 × 103 (2/4)
Vero Cells 48 1.2 × 103 (3/4)
Sand fly Cell Line 48 1.2 × 102 (3/4)
Chick Embryo 48 1.2 × 102 (2/4)
Mice 48 1.2 × 102 (2/4)
Real Time One step RT-PCR - 1.2 × 100 (3/3)
Nested RT-PCR - 1.2 × 100 (3/3)
  1. Limit of detection has been scored positive when ≥ 50% replicates showed CHPV by IFA, plaque formation/death or PCR amplification. Limit of detection has been presented in terms of PFU/ml.