Table 1 Primers and Probe used in the study.
Assay | Primer/Probe | Sequence (5' → 3') | Position |
|---|---|---|---|
P gene | |||
NF5 | Forward Primer | TGAGTGCTCTCCAACTTCTGCAGT | 1682–1705 |
NR5 | Reverse Primer | TTCTTCAGAGCTTGCATCTTGAT | 2331–2309 |
PF1 | Forward Primer | TTTAATCGACATGGGAGCAATTG | 1953–1975 |
PR1 | Reverse Primer | TAAGGTGGGTCAGACGGAGAGA | 2021–2000 |
PP | TaqMan MGB Probe | VIC-AGAATTCATCCTGGCAGCT-NFQ | 1980–1998 |
1st PCR | |||
CHPNDGF3 | Forward Primer | TGATTCCTACATGCCCTATCT | 821–841 |
CHPNDGR3 | Reverse Primer | GAACTTCTTCCCGTTAAGCACG | 1078–1057 |
Nested 2nd PCR | |||
CHPNDGF4 | Forward Primer | TCCACGAAGTCTCCTTACTCT | 863–883 |
CHPNDGR4 | Reverse Primer | GCACGAATCTCTGCTCCAGCT | 1061–1041 |