Figure 3
The invasion suppression assay of antibiotics. A549 cells (1 × 105 cells/well) were infected for 1 h at 37°C with 1 × 107 CFU of YS001 (A) or YS005 (B) in the presence or absence (control) of AMP, AZM, CLR, CFPN, or LVX (1 μg/ml). At 1 h after infection, the cell culture medium was exchanged for fresh medium containing GEN (100 μg/ml). One hour later, surviving intracellular bacteria were enumerated by plating. Data represent the means ± SD of three experiments with triplicate assays. *, P < 0.05 (compared with control). ND, not detected.