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Figure 2 | BMC Infectious Diseases

Figure 2

From: Lysozyme M deficiency leads to an increased susceptibility to Streptococcus pneumoniae-induced otitis media

Figure 2

Compensatory up-regulation of lysozyme P in lysozyme M -/- mice. Conventional RT-PCR shows compensatory up-regulation of lysozyme P when substituting the lysozyme M gene with EGFP in lysozyme M-/- mice (A). Real time quantitative RT-PCR analysis shows that lysozyme P is remarkably up-regulated in the E-tube of lysozyme M-/- mice, compared to the lung (B). ND: not detected. Results were expressed as a fold-expression of mRNA quantity, taking the value of lysozyme P mRNA of the lung as 1. Values are given as mean ± standard deviation. n = 3. *: p < 0.05. The western blot (C) and its densitometry (D) show the expression of lysozyme protein in the E-tube homogenate. As expected, lysozyme M protein (arrow) is depleted and lysozyme P protein (arrow head) is up-regulated in the E-tube of lysozyme M-/- mice, compared to wild type mice. The soluble protein fraction of the E-tube homogenate was separated using 12.5% AU-PAGE electrophoresis, subsequently transferred onto PVDF membranes and labeled with a polyclonal anti-lysozyme antibody (1:5000). Signal was detected by exposure to X-ray film and quantified by the Quantity One® software.

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