Figure 9
From: Activity of the Bacillus anthracis20 kDa protective antigen component
Expression profile of genes related to the Fas pathway that were regulated by rPA 20 in PBMC. Cells were incubated with the rPA20 for 4 hrs. RNA samples were isolated and hybridized on the cDNA micorarray slides as detailed in materials and methods. Images were analyzed using GenePix 4.0 and data were analyzed using GeneSpring 7.0. Data were then analyzed using GeneCite to identify pathways regulated by the rPA20. These genes are caspase 10, Fas (TNFRSF6)-associated via death domain (FADD), p21 (CDKN1A)-activated kinase 2 (PAK2), mitogen-activated protein kinase kinase kinase 7 (MAPKKK7), Rho GDP dissociation inhibitor (GDI) alpha (D4-GDI), ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), retinoblastoma 1 and tumor necrosis factor receptor superfamily, member 6 (FAS).