Gene expression changes linked to antimicrobial resistance, oxidative stress, iron depletion and retained motility are observed when Burkholderia cenocepaciagrows in cystic fibrosis sputum

Table 2 PCR primers and conditions for quantitative PCR

Gene	Forward and reverse primer (5' – 3')	Product size (bp)	Tm (°C)
Real-time PCR
Control gene BCAL1861 (phaC)	AGACGGCTTCAAGGTGGT ACACGGTGTTGACCGTCA	470	62/66
BCAL0270	GCGCGAACCCGATCGAATTC GCCCGCATCCACCAGAAGTG	400	66
BCAM2753	GAACCGCAGACGCTGTACTC AAACGCTCGGTGTTGCGGAC	380	62
BCAL1107	GAATCGACGTATCGGCTCGT ATGATGTGCTTCGGGTTCTTG	377	66
BCAM1676	GACGACCTGTTCCTGCTGTG CGGCTCTTCCTGATGACGTG	534	62
Semi-quantitative PCR
BCAL1165	TGACGCTCGGCACCGTTGAC GCGTGGACCTGCTCGATCTC	400	66

ISSN: 1471-2334