Figure 10

Real-time PCR determination of gene expression in response to each of 8 pathogenic agents. Primers were designed for these 3 genes and 18S, which was used as a reference gene for comparison of these 3 test genes. GBP-2 was a gene that was identified as being massively up regulated by SEB using differential display PCR (Mendis, et al) and was of particular interest to us.