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Table 2 Probe Tms and acceptance criteria

From: Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis

Probe

Melting peak

Mean Tm

SD

N

Tm Acceptance Criteria

136A2

A136

62.0°C

0.49

2228

60.0°C – 64.0°C

136A2

V136

53.2°C

0.36

214

51.2°C – 55.2°C

136A2

T 136

56.9°C

0.15

8

55.2°C – 58.9°C

136A2

AT 137

59.5°C

0.19

7

59.0°C – 60.0°C

154H2

H154

56.6°C

0.51

300

54.6°C – 58.7°C

154H2

R154

49.9°C

0.29

2171

47.9°C – 51.9°C

171R6

R171

55.4°C

0.39

1524

53.4°C – 57.4°C

171R6

Q171

49.8°C

0.31

1392

48.4°C – 51.8°C

171R6

QE 175

49.7°C

0.21

12

48.4°C – 51.8°C

171R6

L 168 Q

47.9°C

0.19

5

46.9°C – 48.4°C

171R6

H171

44.8°C

0.27

168

42.8°C – 46.8°C

171R6

K 171

41.0°C

0.15

8

39.0°C – 42.8°C

141C3

F141

53.9°C

0.19

39

51.9°C – 55.9°C

141C3

L141

45.0°C

0.14

6

43.0°C – 47.0°C

  1. The mean Tms for each probe melting peak are presented, where N is the number of peaks investigated and SD is the standard deviation. The peak Tms for the major PRNP alleles were obtained from six 384 well plates prepared on different working days, thereby accounting for sample-to-sample and run-to-run sources of variation. Variant alleles exhibiting uncommon genotypes or additional SNPs are italicised. Acceptance criteria for these rare polymorphisms were estimated from the few samples analysed, minimising the potential for miscalling melting peaks.