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Table 1 Primer and probe sequences employed for PRNP analysis

From: Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis

Primer/Probe

Sequence (5'-3')

136F2 forward primer

GCAGCTGGAGCAGTGGTAGG

171R2 reverse primer

GATGTTGACACAGTCATGCAC

136A2 probe

GGGAAGFGCCAFGAGCAGGC3

154H2 probe

GACCGFTACTAFCATGAAAACATG3

171R6 probe

CAGTGGAFCGGTAFAGTAAC5AGAA3

141C3 probe

GAGCAGGCCTCTFATAC5FTTTGG3

  1. F and 3 represent fluorescein dT and 3' phosphate respectively. Common SNPs within codons 136, 141, 154 and 171 are underlined. 5 represents the universal base 5-nitroindol employed to neutralise polymorphisms within codons 143 and 175.