Longitudinal monitoring of Ehrlichia ruminantium infection in Gambian lambs and kids by pCS20 PCR and MAP1-B ELISA

Table 2 Periods of detection of E. ruminantium DNA and antibodies in lambs at various age levels sampled in Kerr Seringe

Animal No.	¹Sp.	²Period DNA detected (PCR)							Period antibodies detected (MAP1-B)							Status	⁵ AV
		1	2	3	4	5	6	7	1	2	3	4	5	6	7	Survived
1301	L	-	-	-	-	+	nd	+	+	+	+	-	-	-	-	Survived	0
1302	L	-	-	-	-	+	nd	+	+	+	+	-	-	-	-	Survived
1303	L	-	-	+	-	+	nd	-	+	+	+	-	-	-	-	Survived	0
1304	L	-	-	-	-	-	nd	-	+	+	-	-	-	-	-	Survived
1305	L	-	-	+	+	-	nd	+	+	+	+	-	-	-	-	Survived
1308	L	-	-	-	+	+	nd	-	+	+	+	+	-	-	-	Survived
1309	L	-	-	+	+	+	nd	-	+	+	+	-	-	-	-	Survived
1311	L	-	-	+	-	-	nd	-	+	+	+	+	+	+	+	Survived
1312	L	-	-	-	-	-	nd	-	+	+	+	+	-	-	-	Survived
1313	L	-	-	+	-	+	nd	-	+	+	+	-	-	-	-	Survived
1314	L	-	-	-	-	-	nd	-	+	-	-	-	-	-	-	Survived	0
1315	L	-	+	-	-	-	nd	-	+	nd	+	+	-	-	-	Survived
1316	L	-	-	-	-	-	nd	+	+	-	-	-	-	-	-	Survived
1318	L	-	-	-	nd	+	nd	+	+	+	+	-	-	-	-	Survived
1319	L	-	-	-	-	-	nd	-	+	+	+	+	-	-	-	Survived	0
1320	L	-	-	+	+	+	nd	+	+	nd	+	+	-	-	-	Survived
1322	L	-	-	-	nd	+	nd	-	+	+	+	+	-	-	-	Survived
1323	L	-	-	-	nd	+	nd	-	+	+	+	+	+	+	-	Survived
1325	L	-	-	-	nd	-	nd	-	-	-	-	-	-	-	-	Survived
1327	L	-	-	-	-	-	nd	-	+	+	+	+	-	-	-	Survived
1328	L	-	-	-	nd	-	nd	+	+	+	+	+	-	-	-	Survived
1329	L	-	-	-	nd	-	nd	-	-	-	-	+	-	-	-	Survived
1330	L	-	-	-	-	nd	-	nd	+	+	+	+	-	-	-	Survived
1331	L	-	-	-	-	nd	-	nd	+	+	+	+	-	-	nd	Survived
1333 ⁶	L	nd	+						+	+						³D(28) ⁴hw-G
1332	L	-	-	-					+	+	+					D(42*)
1321	L	-	-	-					+	+	+					D(42*)
1306	L	-	-	-	-				+	+	-	-				D(77*)
1317	L	-	-	-	nd				+	+	+	+				D(84) hw-G	1 N

Key: ¹Sp. = Species; L = lamb; K = kid; ²Age range per level: 1 (0 – 10 days), 2 (11–21 days), 3 (22 – 49 days), 4 (50 – 77 days), 5 (78 – 98 days), 6 (99 – 126), 7 (127 – 162); ³D = died (numbers in parentheses indicate day of death); ⁴hw-G heartwater confirmed by Giemsa; hw-PCR: heartwater confirmed by pCS20 PCR;
⁵ AV: A. variegatum tick counts on selected study animals; N = nymph; A = adult; *mortality due to coccidial and/or clostridial enteritis; ⁶Animal numbers in bold are cases of mortality

ISSN: 1471-2334