BMC Infectious Diseases

Table 5 MTT colorimetric assay of susceptible L (L.) amazonensis

From: Resistance of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisto nitric oxide correlates with disease severity in Tegumentary Leishmaniasis

*L (L.) amazonensis*	NaNO₂ Concentration (mM)
	0	0.25	0.5	1	2	4	8	16
9667	0.488	0.682	0.463	0.373	0.335	0.25	0.027	0.003
9986	0.194	0.278	0.263	0.171	0.197	0.049	0.01	0.002
*L. (V.) braziliensis*	NaNO₂ Concentration (mM)
	0	0.25	0.5	1	2	4	8	16
11155	0.389	0.478	0.488	0.432	0.113	0.025	0.007	0.004
13396	0.292	0.269	0.28	0.325	0.224	0.038	0.012	0.009
13323	0.407	0.265	0.382	0.684	0.228	0.157	0.033	0.014
Lb 001	0.249	0.178	0.191	0.167	0.169	0.125	0.091	0.03
9291	0.628	0.645	0.437	0.253	0.101	0.033	0.051	0.02
13690	0.558	0.646	0.448	0.326	0.13	0.055	0.023	0.073
14183	0.178	0.166	0.168	0.096	0.048	0.035	0.017	0.009
9139	0.446	0.277	0.221	0.305	0.182	0.093	0.047	0.012
13183	0.598	0.39	0.38	0.373	0.02	0.036	0.014	0.007
13548	0.547	0.58	0.894	0.584	0.382	0.246	0.053	0.032
14349	0.812	0.556	0.426	0.404	0.208	0.108	0.085	0.086
14808	0.261	0.231	0.251	0.160	0.096	0.074	0.024	0.08

1 × 10⁶ L. (L.) amazonensis and L. (V.) braziliensis promastigotes contained were exposed for 4 hours to increasing concentrations of the NO^• donor NaNO₂ (0.25–16 mM) in HBSS pH 5.0. The cells were then washed and distributed in parasite growth medium for 20 hours. After this time the supernatants were removed and the parasites were incubated in HBSS, pH 7.0 plus 10 μl of MTT for 4 hours. Viability was a measured by the conversion of MTT to formazan and is expressed as the OD at 540 nm. Data are mean ± SD of NO^•- resistant Leishmania isolates (Table 4) or NO^• susceptible Leishmania isolates (Table 5).

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ISSN: 1471-2334

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