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Figure 1 | BMC Infectious Diseases

Figure 1

From: Resistance of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisto nitric oxide correlates with disease severity in Tegumentary Leishmaniasis

Figure 1

Survival of L. (V.) braziliensis and L.(L.) amazonensis promastigotes in increasing concentrations of NO measured by Thymidine incorporation (A and B) and colorimetric MTT assay (C and D). Promastigotes in HBSS pH 5.0 were exposed to increasing NaNO2 concentrations (0.25–16 mM) for 4 hours. Viability was measured by incorporation of 3H-TdR or conversion of MTT of formazan as described in the Methods section. Data in panels A and B represent mean ± SEM percentage survival for (1A) 8 resistant L. (L.) amazonensis and two resistant L. (V.) braziliensis isolates, (1B) two susceptible L. (L.) amazonensis and 4 susceptible L. (V.) braziliensis isolates as measured by thymidine incorporation. Data in panels C and D represent the mean ± SEM MTT conversion for (1C) 3 NO- resistant L. (L.) amazonensis and 2 NO-resistant L. (V.) braziliensis isolates, or (1D) 2 susceptible L. (L.) amazonensis and 12 susceptible L. (V.) braziliensis isolates.

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