Table 1 Comparison of various identification methods for 4 ATCC reference strains of Lactobacillus, Pediococcus, Leuconostoc, and 26 catalase-negative, gram-positive clinical isolates from 1997–2004 (numbers 1–26) initially identified as Leuconostoc by API 20 STREP or 7 isolates from 2005–2006 expressing high levels of vancomycin resistance (numbers 31–33 and 35–38).
Isolate #-specimen type | API 20 STREP (% identity) | API 50 CHL (% identity) | Gram's staining results | ADH | LAP | MRS | PYR | Van | PCR for Lactobacillus and Leuconostoc | Sequencing of 16S rDNA gene (% identity) |
|---|---|---|---|---|---|---|---|---|---|---|
Lactobacillus plantarum ATCC 14917* | Enterococcus avium (63.2) | Lactobacillus plantarum (99.9) | B | -/- | +/+ | - | -/- | R | +ve Lactobacillus | Lactobacillus plantarum (100) |
Lactobacillus pentosus ATCC 8041* | Leuconostoc (81.1) | Lactococcus lactis ssp lactis 1 (82.5) | B | -/- | +/+ | + | -/- | R | +ve Lactobacillus | N/A |
Pediococcus pentosaceus ATCC 33316* | Leuconostoc (39.3) | Lactobacillus pentosus (84.3) | C | +/+ | +/+ | - | -/- | R | Neg | Pediococcus pentosaceus (99.4) |
Leuconostoc mesenteroides ATCC 8293 | Leuconostoc (96.8) | Leuconostoc mesenteroides ssp mesenteroides/dextranicum 1 (95.7) | Cb | -/- | -/- | + | -/- | R | +ve Leuconostoc | Leuconostoc mesenteroides (99.3) |
1-pus* | Streptococcus suis biotype I (85.6) | Lactobacillus acidophilus (97.4) | C-Ch | -/+# | +/-# | - | -/- | S | Neg | Streptococcus suis (100) |
2-blood* | Leuconostoc (99.8) | Lactococcus lactis ssp lactis 1 (90.5) | Cb | +/+ | -/- | + | -/- | R | Neg | N/A |
3-corneal discharge | Leuconostoc (97.9) | Lactobacillus brevis 3 (98.8) | C-Ch | -/- | -/- | + | -/- | R | +ve Leuconostoc | N/A |
4-ascitic fluid | Leuconostoc (99.9) | Leuconostoc mesenteroides spp mesenteroides/dextranicum 2 (99.9) | C | -/- | -/- | + | -/- | R | +ve Leuconostoc | Leuconostoc lactis or garlicum (99.5) |
5-ascitic fluid* | Leuconostoc (93.6) | Lactobacillus acidophilus 1 (85.1) | Cb | +/+ | -/- | + | -/- | R | Neg | N/A |
6-blood | Leuconostoc (95.4) | Leuconostoc lactis (96.0) | Cb | -/- | -/- | + | -/- | R | +ve Leuconostoc | Leuconostoc lactis or garlicum (99.6) |
7-blood | Leuconostoc (68.5) | Leuconostoc lactis (92.0) | Cb | -/- | -/- | + | -/- | R | Neg | N/A |
8-blood* | Streptococcus mitis 1 (81.1) | Lactobacillus acidophilus (92.1) | Cb | -/- | +/-# | - | -/- | S | Neg | N/A |
9-blood* | Leuconostoc (97.2) | Leuconostoc mesenteroides spp cremoris (99.9) | Cb | +/+ | -/- | + | -/- | R | Neg | Weissella cibaria (100) |
10-blood* | Abiotrophia adiacens (46.9) Aerococcus viridans 2 (27.5) | Lactobacillus delbrueckii spp delbrueckii (78.1) | Cb | -/- | -/- | - | -/- | S | Neg | N/A |
11-blood* | Leuconostoc (92.2) | Lactobacillus acidophilus (72.5) | C-Ch | -/- | -/+# | - | -/- | S | Neg | N/A |
12-ascitic fluid* | Lactococcus lactis spp cremoris (47.2) Leuconostoc (45.1) | Lactobacillus salivarius (99.9) | C-Ch | -/- | -/- | - | -/- | R | +ve Lactobacillus | N/A |
13-blood* | Streptococcus sanguis (49.2) other streptococci (48.5) | Leuconostoc mesenteroides spp cremoris (98.7) | Cb | -/- | +/-# | - | -/- | S | Neg | N/A |
14-blood* | Leuconostoc (39.0) Lactococcus lactis ssp cremoris (37.9) | Lactobacillus acidophilus 1 (88.2) | C | -/- | -/+# | - | -/- | S | Neg | Streptococcus pasteurianus (99.9) |
15-blood* | Streptococcus bovis (64.8) | Leuconostoc lactis (87.9) | Cb | -/- | +/-# | - | -/- | R | Neg | Weissella confusa (99.9) |
16-blood* | Enterococcus faecium (98.7) | Lactobacillus plantarum 1 (98.6) | Cb | +/+ | +/-# | - | +/-# | S | Neg | Enterococcus faecium (99.9) |
17-blood* | Aerococcus viridans (62.6) | Lactobacillus delbrueckii spp delbruekii (95.5) | Cb | -/- | -/- | - | -/- | S | Neg | Actinomyces odontolyticus (98.9) |
18-brain abscess* | Streptococcus constellatus (99.9) | Lactobacillus acidophilus (82.1) | C-Ch | +/+ | +/-# | + | -/- | S | Neg | Streptococcus anginosus or constellatus (99.7) |
19-blood* | Streptococcus bovis biotype II (64.8) | Lactobacillus acidophilus 1 (98.1) | C-Ch | -/- | +/-# | + | -/- | S | Neg | Streptococcus constellatus (99.7) |
20-blood* | Leuconostoc (92.2) | Weissella viridescens (99.8) | C | -/+# | -/+# | - | -/- | R | Neg | Weissella viridescens (99.9) |
21-lung swab* | Leuconostoc (99.9) | Lactobacillus acidophilus (78.4) | Cb | +/+ | -/- | + | -/- | R | Neg | Weissella cibaria (100) |
22-bone* | Leuconostoc (99.6) | Lactobacillus coprophilus (96.9) | Cb | +/+ | -/- | + | -/- | R | Neg | Weissella confusa (99.61) |
23-NR* | Leuconostoc (98.8) | Lactobacillus coprophilus (96.9) | Cb | +/+ | -/+# | - | -/+# | R | Neg | Weissella confusa (100) |
24-NR* | Aerococcus viridans (48.5) Lactococcus lactis ssp cremoris (41.6) | Leuconostoc mesenteroides spp cremoris (94.7) | C-Ch | -/+# | +/-# | - | -/- | S | Neg | Streptococcus anginosus (99.9) |
25-pus* | Streptococcus constellatus (99.9) | Lactobacillus delbrueckii (80.4) | C-Ch | +/+ | +/-# | - | -/- | S | Neg | Streptococcus constellatus (99.7) |
26-duodenal* content | Leuconostoc (89.9) | Lactobacillus salivarius (99.9) | Cb | -/- | +/-# | - | -/- | R | +ve Lactobacillus | Lactobacillus salivarius (100) |
31-urine* | Leuconostoc (99.7) | Lactobacillus acidophilus 1 (49.5) | Cb | +/+ | -/- | + | -/- | R | Neg | Weissella cibaria (99.5) |
32-gastric content | Leuconostoc (99.4) | Leuconostoc lactis (95.3) | C-Ch | -/- | -/- | + | -/- | R | +ve Leuconostoc | Leuconostoc garlicum or lactis (99.2) |
33-tissue biopsy | Leuconostoc (99.9) | Leuconostoc mesenteroides spp mesenteroides/dextranicum2 (99.9) | C-Ch | -/- | -/- | + | -/- | R | +ve Leuconostoc | Leuconostoc garlicum or lactis (99.6) |
35-ascitic fluid* | Leuconostoc (65.8) | Pediococcus pentosaceus 1 (63.6) | C | -/- | +/+ | - | -/- | R | neg | Pediococcus stilesii (91.5) |
36-blood | Leuconostoc (92.8) | Lactobacillus collinoides or fermentum 1 (98.3) | Cb | -/- | -/- | + | -/- | R | neg | Weissella confusa (99.9) |
37-pleural fluid* | Leuconostoc (69.2) | Lactobacillus salivarius (99.9) | Cb | -/- | +/+ | - | -/- | R | neg | N/A |
38-tissue biopsy* | Leuconostoc (82.7) | Pediococcus pentosaceus (99.9) | C | +/+ | +/+ | - | -/- | R | neg | Pediococcus pentosaceus (98.2) |