BMC Infectious Diseases

Table 3 Sensitivity and specificity of antibody staining techniques (monoclonal and polyclonal IHC) for the detection of WNV in avian tissue samples, using RT-PCR as a gold standard (n = 85, 43 positive, 42 negative by RT-PCR)

From: Sensitivity and specificity of monoclonal and polyclonal immunohistochemical staining for West Nile virus in various organs from American crows (Corvus brachyrhynchos)

		# of Samples			Sensitivity		Specificity
		All	Pos	Neg	Pt. Est.	95% C.I.	Pt. Est.	95% C.I.
Overall*	Monoclonal	85	31	54	72.1	57.3 – 83.3	100	91.6 – 100
	Polyclonal	85	43	42	100	91.8 – 100	100	91.6 – 100
Heart	Monoclonal	79	20	59	47.6	33.4 – 62.3	100	90.6 – 100
	Polyclonal	79	42	37	100	91.6 – 100	100	90.6 – 100
Kidney*	Monoclonal	73	25	48	62.5	47.0 – 75.8	100	89.6 – 100
	Polyclonal	73	39	34	97.5	87.1 – 99.6	100	89.6 – 100
Brain*	Monoclonal	54	5	49	29.4	13.3 – 53.1	100	90.6 – 100
	Polyclonal	54	6	48	35.3	17.3 – 58.7	100	90.6 – 100
Spleen	Monoclonal	38	10	28	66.7	41.7 – 84.8	100	85.7 – 100
	Polyclonal	37	14	23	93.3	70.2 – 98.8	100	85.1 – 100
Liver	Monoclonal	50	10	40	66.7	41.7 – 84.8	100	90.1 – 100
	Polyclonal	50	15	35	100	79.6 – 100	100	90.1 – 100
Lung	Monoclonal	40	10	30	76.9	49.7 – 91.8	100	87.5 – 100
	Polyclonal	42	13	29	100	77.2 – 100	100	88.3 – 100
Small Intestine	Monoclonal	50	15	35	83.3	60.8 – 94.2	100	89.3 – 100
	Polyclonal	53	18	35	100.0	82.4 – 100	100.0	90.1 – 100

* Indicates statistically significant difference between monoclonal and polyclonal tests, Fisher's Exact p ≤ 0.05

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