Table 1 Details of oligonucleotide sequences used for detection of C. pneumoniae by NAATs.

1.

16SrRNA

Multiplex

C.pneumoniae

5' TGA CAA CTG TAG AAA TAC AGC 3'

463 bp

30

5' TAA ATA TCC TCT CTC CGC 3'

C.psittaci

5' TGA CCG CGG CAG AAA TGT CGT 3'

5' GAC GTT TCC TCT CTC CGC 3'

C.trachomatis

5' TGA CCG CGG CAG AAA TGT CGT 3'

5'GGC GTT CCC TCT CTC CGC 3'

2.

Omp1

Semi-nested

(1^st pair)

5' TGC CAA CAG ACG CTG GCG T 3'

35

5' TCT GAA CTG ACC AGA TAC GT 3'

(2^nd pair)

5' TGC GAC CAT CAA TTC TCA TG 3'

360 bp

30

5' TCT GAA CTG ACC AGA TAC GT 3'

3.

16SrRNA

Nested

(1^st pair)

5' GCT GGC GGC GTG GAT G 3'

30

5' CGA CAC GGA TGG GGT TG 3'

(2^nd pair)

5' TGG CGG AAG GGT TAG TAG TA 3'

570 bp

30

5' CCC TTT TCC CCA TCT ATC C 3'