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Table 3 Sensitivity, specificity, positive and negative predictive values of plasma CMV quantitative PCR

From: CMV quantitative PCR in the diagnosis of CMV disease in patients with HIV-infection – a retrospective autopsy based study

Results of CMV PCR assay Sensitivity (%) (95% CI) Specificity (%) (95% CI) PPV (%) (95% CI) NPV (%) (95% CI)
Viral load (CMV DNA copies/mL (last samples)     
Viraemiaa 47 (34–60) 89 (80–94) 76 (59–87) 70 (60–78)
   ≥2 000 30 (20–44) 96 (89–99) 84 (62–95 65 (56–74)
   ≥10 000 17 (9–29) 100 (95–100) 100 (70–100) 62 (53–70)
CMV DNA detectiona by no. of tested samples before diagnosis     
   One sample     
CMV DNA detected 46 (30–65) 90 (69–97) 85 (58–96) 57 (39–73)
   Two samples     
CMV DNA detected in at least one sample 55 (38–72) 86 (74–92) 67 (47–82) 78 (66–87)
CMV DNA detected in both samples 24 (12–42) 100 (94–100) 100 (65–100) 70 (61–80)
  1. aAny viraemia above limit of detection.
  2. Sensitivity, specificity, positive and negative predictive value of plasma CMV quantitative PCR in the diagnosis of CMV disease in HIV patients by different viral load cut-offs and number of tested samples.