Figure 2

Effect of Sp110 on A. phagocytophilum infection of human HL-60 cells. A. phagocytophilum msp4 DNA levels were determined by real-time PCR in infected HL-60 cells with knockdown Sp110, PSGL-1 (positive control) and ARP3 (negative control). A. phagocytophilum msp4 DNA levels were normalized against human Alu sequences and infection levels were compared between cells nucleofected with Sp110 or PSGL-1 siRNAs and control cells treated with ARP3 siRNA by Student's t-Test (*P < 0.05; N = 8).