Table 1 Primers, probes and restriction enzymes
Gene | SNP | Sense primer | Antisense primer | Probe/restriction enzyme |
|---|---|---|---|---|
TNF-α | G- 308 A | 5'-TAGGTTTTGAGGGGCATGGGGAC-3' | 5'- TCTCGGTTTCTTCTCCATCG -3' | 5'-TAGGTTTTGAGGGGCATGGGGAC-3'-fluorescein 5'-LC Red640-GGGTTCAGCCTCCAGGGTCCTACACAC-3'-phosphate |
Il-1β | C3953T | 5'-CACTCCCAGCTTCATCCCTA-3' | 5'-AGGTGCATCGTGCACATAAG-3' | Taq I |
PAI-1 | 4G/5G | 5G allele; 5 ' -GTCTGGACACGTGGGGG-3 ' 4G allele; 5 ' -GTCTGGACACGTGGGGA-3 ' | 5 ' -TGCAGCCAGCCACGTGATTGTCTAG-3 ' | None |
uPA | C 422 T | 5'-biotin-ACTGCAGGAACCCAGACAAC-3' | 5'-AGGGAGGCAGGTAGGAGAAA-3' | 5'-GCCTAAAGCCGCTTGTCCAA-3' 5'-GGCCTAAAGCTGCTTGTCCAA-3' |
TLR4 | A 896G | 5'-biotin-AGTCCATCGTTTGGTTCTGG-3' | 5'-AATAGTCACACTCACCAGGGAAA-3' | 5'-AGTCAATAATATCATCGAGGTAG-3' 5'-AGTCAATAATACCATCGAGGTAG-3' |
CD14 | C-159T | 5'-biotin-CACCCACCAGAGAAGGCTTA-3' | 5'-ATCACCTCCCCACCTCTCTT-3' | 5'-GGAGGGGGGCCGTAACA-3' 5'-GGGAGGGGGACCGTAACAG-3' |