Real time PCR melting curve and agarose gel electrophoresis results obtained from saliva reconstruction samples containing different
genotypes. Curves 1–2: using a suspension containing the 652 or JP2 Aa genotype, the difference in Tms melting peaks was 6°C between the two clones. The presence of a single 696 bp band for 652 and 192 bp for the JP2 strain in 1% agarose electrophoresis gel (Metaphor, BMA, Rockland ME USA) suggests a total absence of non-specific products, gel bands 1–2. Melting peak at 73°C in negative control and the small band in the control samples "C" are primer dimer products.