Figure 2From: Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva Real time PCR melting curve and agarose gel electrophoresis results obtained from saliva reconstruction samples containing different Actinobacillus actinomycetemcomitans genotypes. Curves 1–2: using a suspension containing the 652 or JP2 Aa genotype, the difference in Tms melting peaks was 6°C between the two clones. The presence of a single 696 bp band for 652 and 192 bp for the JP2 strain in 1% agarose electrophoresis gel (Metaphor, BMA, Rockland ME USA) suggests a total absence of non-specific products, gel bands 1–2. Melting peak at 73°C in negative control and the small band in the control samples "C" are primer dimer products.Back to article page