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Figure 2 | BMC Infectious Diseases

Figure 2

From: Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva

Figure 2

Real time PCR melting curve and agarose gel electrophoresis results obtained from saliva reconstruction samples containing different Actinobacillus actinomycetemcomitans genotypes. Curves 1–2: using a suspension containing the 652 or JP2 Aa genotype, the difference in Tms melting peaks was 6°C between the two clones. The presence of a single 696 bp band for 652 and 192 bp for the JP2 strain in 1% agarose electrophoresis gel (Metaphor, BMA, Rockland ME USA) suggests a total absence of non-specific products, gel bands 1–2. Melting peak at 73°C in negative control and the small band in the control samples "C" are primer dimer products.

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