Figure 1From: A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive controlStandard curve of the matrix gene real-time RRT-PCR assay. Serial 10-fold dilutions of in vitro transcribed matrix RNA standard (from 1 to 10 8 copies/μl) were plotted against the threshold cycle. Each plot represents the mean of 10 replicate amplifications of each dilution. The coefficient of determination (R2) and the equation of the regression curve (y) calculated.Back to article page