Figure 1

IFN-γ and TNF-α production induced by antigens from L. braziliensis isolates isolated CL or DL cases. PBMCs from cutaneous leishmaniasis (CL) (hatched bars, n = 24) and disseminated leishmaniasis patients (black bars, n = 15) were stimulated with antigens of L. braziliensis genotyped isolate from cutaneous leishmaniasis (Ag-CL) and disseminated leishmaniasis (Ag-DL). Data represent mean ± standard error mean (SEM) of supernatants collected and assayed by ELISA for (A) IFN-γ, *P < 0.02 and **P < 0.01, Mann-Whitney test; ⁺P < 0.005 and ⁺⁺P < 0.001, Wilcoxon paired non-parametric test; and for (B) TNF-α, *P < 0.03 and **P < 0.8, Mann-Whitney test; ⁺, ⁺⁺P < 0.001, Wilcoxon paired non-parametric test.