a Combined average of IκB levels from cells treated with 7 low-binders or 7 high-binders. Each S. pneumoniae isolate was tested in triplicate thus each measurement is the average from 21 different experiments at each time point.
b A549 cells were incubated with an MOI of 50 bacteria/cell for the listed time periods. Proteins from the cell culture were then harvested for the analysis of IκB degradation.
c Two-way analysis of variance comparing IκB degradation in A549 treated with low binders and high binders for each time period. IκB degradation measured as described in materials and methods.