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Table 2 Percentage of IκB remaining in A549 after addition of high- and low-binding S. pneumoniae a

From: Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

  Time after addition of bacteria
Treatment of A549 cellsb 0 hours 4 hours 6 hours 8 hours
low binders 100% 81.3% 65.6% 62.1%
high binders 100% 60.8% 34.9% 25.1%
ANOVA p-valuec 0.423 2.3 × 10-7 1.3 × 10-7 1.1 × 10-8
  1. a Combined average of IκB levels from cells treated with 7 low-binders or 7 high-binders. Each S. pneumoniae isolate was tested in triplicate thus each measurement is the average from 21 different experiments at each time point.
  2. b A549 cells were incubated with an MOI of 50 bacteria/cell for the listed time periods. Proteins from the cell culture were then harvested for the analysis of IκB degradation.
  3. c Two-way analysis of variance comparing IκB degradation in A549 treated with low binders and high binders for each time period. IκB degradation measured as described in materials and methods.