Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

Table 2 Percentage of IκB remaining in A549 after addition of high- and low-binding S. pneumoniae ^a

	Time after addition of bacteria
Treatment of A549 cells^b	0 hours	4 hours	6 hours	8 hours
low binders	100%	81.3%	65.6%	62.1%
high binders	100%	60.8%	34.9%	25.1%
ANOVA p-value^c	0.423	2.3 × 10^-7	1.3 × 10^-7	1.1 × 10^-8

^a Combined average of IκB levels from cells treated with 7 low-binders or 7 high-binders. Each S. pneumoniae isolate was tested in triplicate thus each measurement is the average from 21 different experiments at each time point.
^b A549 cells were incubated with an MOI of 50 bacteria/cell for the listed time periods. Proteins from the cell culture were then harvested for the analysis of IκB degradation.
^c Two-way analysis of variance comparing IκB degradation in A549 treated with low binders and high binders for each time period. IκB degradation measured as described in materials and methods.

ISSN: 1471-2334