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Table 3 Detection and quantitation of GI/GII mixtures using the multiplex real-time RT-PCR

From: Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe

  Expected quantity (copies/assay) Detected quantity (copies/assay)
  GI GII GI GII
plasmid DNA 106 106 1.2 × 106 1.4 × 106
  104 104 2.2 × 104 1.8 × 104
  102 102 2.6 × 101 5.1 × 100
viral RNA 106 106 3.3 × 106 4.7 × 106
  105 105 4.0 × 105 3.2 × 105
  104 104 4.4 × 104 2.7 × 104
  102 102 6.0 × 103 5.0 × 100
  1. Known copy numbers of GI and GII plasmid DNA and GI and GII viral RNA were mixed and analyzed by the multiplex real-time RT-PCR