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Table 3 Detection and quantitation of GI/GII mixtures using the multiplex real-time RT-PCR

From: Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe

 

Expected quantity (copies/assay)

Detected quantity (copies/assay)

 

GI

GII

GI

GII

plasmid DNA

106

106

1.2 × 106

1.4 × 106

 

104

104

2.2 × 104

1.8 × 104

 

102

102

2.6 × 101

5.1 × 100

viral RNA

106

106

3.3 × 106

4.7 × 106

 

105

105

4.0 × 105

3.2 × 105

 

104

104

4.4 × 104

2.7 × 104

 

102

102

6.0 × 103

5.0 × 100

  1. Known copy numbers of GI and GII plasmid DNA and GI and GII viral RNA were mixed and analyzed by the multiplex real-time RT-PCR
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BMC Infectious Diseases

ISSN: 1471-2334

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