Table 2 Detection of norovirus of different genotypes by multiplex real-time-PCR. Comparison to numbers of NV positive specimens as tested by nested PCR/monoplex real-time PCR
Genotype* | Prototype strain | GenBank no. | Detection by multiplex TaqMan (no. pos./no. tested |
|---|---|---|---|
I.1 | Norwalk//1968/USA | M87661 | 1/1 |
I.2 | Southampton/1991/UK | L07418 | 2/2 |
I.3 | Desert Shield395/1990/SA | U04469 | 7/7 |
I.6 | Sindlesham/1995/UK | AJ277615 | 2/2 |
II.2 | Melksham/1989/UK | X81879 | 2/3 |
II.3** | Arg320/1995/AR | AF190817 | 4/4 |
II.4 | Grimsby-like 2002 | AY485642 | 3/3 |
II.4 | Grimsby-like 2004 | AY883096 | 60/62 |
II.7 | Leeds/1990/UK | AJ277608 | 9/9 |
II.10 | Erfurt/546/2000/GE | AF427118 | 1/1 |
II.unclassified | distantly related to | AY673935 | 2/2 |
II.unclassified | distantly related to | AB084786 | 1/1 |
II. not determ. | 3/3 | ||
summary | 97/100 (97 %) | ||
NV negative | 0/40 (100 %) |