Table 1 Primers and probes used for one tube multiplex real-time RT-PCR
Genogroup | Primer | Sequence (5' – 3') | Location* |
|---|---|---|---|
G I | NV192 (s) | 5'-GCYATGTTCCGCTGGATGC | 5282–5300 |
NV193 (as) | 5'-CGTCCTTAGACGCCATCATCA | 5379–5359 | |
TM9-MGB probe | 5'-VIC-TGGACAGGAGATCGC-MGB-NFQ | 5345–5359 | |
G II | NV107a (s) | 5'-AGCCAATGTTCAGATGGATG | 5007–5026 |
NV107c (s) | 5'-AICCIATGTTYAGITGGATG | 5007–5026 | |
NV119 (as) | 5'-TCGACGCCATCTTCATTCAC | 5100–5081 | |
TM3A probe | 5'-6'FAM-TGGGAGGGCGATCGCAATCTGGC-NFQ | 5048–5070 |