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Table 1 Primers and probes used for one tube multiplex real-time RT-PCR

From: Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe

Genogroup Primer Sequence (5' – 3') Location*
G I NV192 (s) 5'-GCYATGTTCCGCTGGATGC 5282–5300
  NV193 (as) 5'-CGTCCTTAGACGCCATCATCA 5379–5359
  TM9-MGB probe 5'-VIC-TGGACAGGAGATCGC-MGB-NFQ 5345–5359
G II NV107a (s) 5'-AGCCAATGTTCAGATGGATG 5007–5026
  NV107c (s) 5'-AICCIATGTTYAGITGGATG 5007–5026
  NV119 (as) 5'-TCGACGCCATCTTCATTCAC 5100–5081
  TM3A probe 5'-6'FAM-TGGGAGGGCGATCGCAATCTGGC-NFQ 5048–5070
  1. *Genome location of primers and probes are based for GI on the sequence of Norwalk/68/US [GenBank: M87661] and for GII on the sequence of Lordsdale/93/UK [GenBank: X86557]; Y= C/T; I= inosin; MGB= minor groove binder; NFQ= non fluorescent quencher