Paired recruitment and 12 months quantitative counts of Spot Forming Units (SFU)/2 × 105 cells in ELISPOT tests using CFP-10 (Fig. 2a), E-SAT6 (Fig. 2b) and PPD (Fig. 2c) as stimulatory antigens for cured, HIV-negative patients (n = 82). Filled black squares (■) represent the SFU count at recruitment; white squares (□) represent SFU counts 12 months later, after successful completion of TB treatment. The change in counts between the two timepoints is represented by the linking vertical bar. With all three stimulatory antigens, the average within-subject decline in SFU counts is highly significantly (p < 0.001).