Figure 4
From: Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection
LightCycler™ amplification profile of the IS481 MB assay with Bordetella isolates The samples correspond to: 1 = B. bronchiseptica ATCC 10580, 2 = B. bronchiseptica ATCC 29513, 3 = B. parapertussis ATCC 1533, 4 = B. parapertussis 33047, 5 = B. parapertussis 33205, 6 = B. holmesii ATCC 51541, 7 = B. holmesii 98P1995-F, 8 = B. holmesii 98P0971-D, 9 = B. pertussis ATCC 9797. The specific amplified product is 154 bp and the agarose gel of products is given as an inlay (numbers correspond to samples in the amplification curves). Stocks of Bordetella isolates were approximately 106cfu/ml unless otherwise quoted. MB = molecular beacon