Figure 2
From: Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection
LightCycler™ amplification profile of the IS481 HP probe assay with a dilution series of B. pertussisATCC 9797 strain of B. pertussis was utilized (106 cfu/ml to 10-3 cfu/ml). Analytical sensitivity of this assay is 10 cfu/ml (0.1 cfu input). The dashed lines correspond to the average Ct value where conventional results are: 1 = DFA positive and culture positive, 2 = DFA negative, culture positive, 3 = DFA negative, culture negative. HP = hybridization probe, Ct = crossing threshold, DFA = direct fluorescent antigen