Human T-lymphotropic virus type 1 (HTLV-1) prevalence and quantitative detection of DNA proviral load in individuals with indeterminate/positive serological results

Table 2 Intra-assay and inter-assay analysis of Ct mean value of standard curves obtained with scalar dilution of MT2 cell line (from 10⁵ to 1).

MT₂ cell line N° copies/reaction	Ct mean values ± SD	CV
Intra-assay^a
10⁵	23.35 ± 0.7	3.0
10⁴	25.91 ± 0.8	3.1
10³	28.10 ± 1.0	3.5
10²	31.78 ± 1.2	3.7
10	36.18 ± 1.3	3.5
1	ND	-
Inter-assay ^b
10⁵	23.90 ± 0.7	2.9
10⁴	26.12 ± 0.9	3.4
10³	28.30 ± 1.1	3.8
10²	32.10 ± 1.3	4.0
10	36.50 ± 1.4	3.8
1	ND	-

SD : standard deviation; CV: coefficient of variation; ND: not detectable
^a For each sample, the Ct value is the average of results from three replicates.
^b For each sample, the Ct value is the average of results from three different experiments performed in triplicate.

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