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Table 2 Intra-assay and inter-assay analysis of Ct mean value of standard curves obtained with scalar dilution of MT2 cell line (from 105 to 1).

From: Human T-lymphotropic virus type 1 (HTLV-1) prevalence and quantitative detection of DNA proviral load in individuals with indeterminate/positive serological results

MT2 cell line N° copies/reaction Ct mean values ± SD CV
Intra-assaya   
105 23.35 ± 0.7 3.0
104 25.91 ± 0.8 3.1
103 28.10 ± 1.0 3.5
102 31.78 ± 1.2 3.7
10 36.18 ± 1.3 3.5
1 ND -
Inter-assay b   
105 23.90 ± 0.7 2.9
104 26.12 ± 0.9 3.4
103 28.30 ± 1.1 3.8
102 32.10 ± 1.3 4.0
10 36.50 ± 1.4 3.8
1 ND -
  1. SD : standard deviation; CV: coefficient of variation; ND: not detectable
  2. a For each sample, the Ct value is the average of results from three replicates.
  3. b For each sample, the Ct value is the average of results from three different experiments performed in triplicate.