Figure 2
Agarose gel electrophoresis and Southern blot of samples amplified by SYBR Green real time PCR assay. In the upper part of the figure: the two positive HTLV-I samples (lanes 1 and 11), the samples with HTLV-I indeterminate or positive serological assays (lanes 2–10), MT-2 representative cells scalar dilutions (from 102 to 104; lanes 12–14) and molecular weight markers (lane 15) are shown. The bottom of the figure shows the Southern blot assay. The non-specific bands exhibited in the HTLV-I negative samples (lane 7–9) represent non-specific products that both did not hybridize with the HTLV-I specific internal probe and showed an unrelated melting temperature in SYBR Green real time PCR.