Figure 6From: Detection of Chlamydia in the peripheral blood cells of normal donors using in vitroculture, immunofluorescence microscopy and flow cytometry techniques Detection, identification and quantification of Chlamydia infected cells in buffy coat pools from FB samples using flow cytometry. Fresh blood smears were assessed for Chlamydia-positive cells by immunostain. Aliquots of the pooled BC were dual immunostained to detect intracellular Chlamydia and identify CD16b, CD14, or CDSw125 cells. For each of the dual-immunostained BC pairs, aliquots of 10,000 cells were assessed. Left panels: results for the weakly positive pool. Right panels: results for the positive pool. The dot plots indicate: in vivo each cell type can be infected with Chlamydia, the different cell-types exhibit different patterns with different percentages of Chlamydia-infected cells and inherently the same preparation provides enumeration of uninfected as well as infected cells, thereby providing numerical totals for infection of each cell type/10,000 cells.Back to article page