Detection, identification and quantification of
infected cells in buffy coat pools from FB samples using flow cytometry. Fresh blood smears were assessed for Chlamydia-positive cells by immunostain. Aliquots of the pooled BC were dual immunostained to detect intracellular Chlamydia and identify CD16b, CD14, or CDSw125 cells. For each of the dual-immunostained BC pairs, aliquots of 10,000 cells were assessed. Left panels: results for the weakly positive pool. Right panels: results for the positive pool. The dot plots indicate: in vivo each cell type can be infected with Chlamydia, the different cell-types exhibit different patterns with different percentages of Chlamydia-infected cells and inherently the same preparation provides enumeration of uninfected as well as infected cells, thereby providing numerical totals for infection of each cell type/10,000 cells.